If a dsDNA molecule is cut by two restriction enzymes (I and II) , three fragments of DNA will be generated A,B and C . Which fragment will be separated the farthest from the well during gel electrophoresis ?

Which procedure is employed to separate the DNA fragments formed by restriction enzymes?

In the process of rDNA technology, if two separate restriction renzymes are used to cut vector and donor DNA then which problem will arise in the formation of rDNA or chimeric DNA. Explain.

Information about protein synthesis is stored in the DNA. Proteins are synthesized by DNA through RNA. This is also known as the central dogma of life. The nucleotide sequences of the mRNA produced are complementary to the DNA strand that is uded as the template for synthesis. This process of synthesis of RNA from DNA is known as transcription. The code for each amino acid consists of three nucleotides (triplet codon) that are present on the mRNA. The tRNA has an anticodon sequence complementary to the cofon on the mRNA. During translation, the code on mRNA is read nad respective amino acids brought by tRNA are joined together by peptide bonds. Based on the given paragraph, answer the followig question: i. If 3'-AACGT-5' is a sequence of the template DNA strand, what would be the nucleotide sequence of the corresponding mRNA synthesized from it? ii. What is the difference in nitrogeneous bases of DNA and RNA? iii. Which enzyme woud be required for the synthesis of RNA from DNA during transcription? iv. How many amino acids can the following mRNA sequence code for ? 5'-UUCAGCCGUGUCAUU-3' v. What is the function of mRNA in translation?

Given below are four statements pertaining to separation of DNA fragments using gel electrophoresis. Identify the incorrect statements. (i) DNA is negatively charged molecule and so it is loaded on gel towards the Anode terminal. (ii) DNA fragments travel along the surface of the gel whose concentration does not affect movement of DNA. (iii) Smaller the size of DNA fragment, larger is the distance it travels through it. (iv) Pure DNA can be visualized directly by exposing to UV radiation. Choose correct answer from the options given below :

The figure shows the elliptical orbit of a planet about the sun S. An ellipse is the curve obtained when a cone is cut by an inclined plane. It has two focal points. The sum of the distances to the two focal points from every point on the curve is constant. F_1 " and " F_2 are the two focal points of the ellipse . The shaded area CF_1D is twice the shaded area AF_1B. t_1 is the time taken by the planet to move from C to D and t_2 is the time to move from A to B Which laws do we understand from the above diagram and description?

Read the following statements and find out the incorrect statement. A. Ultimately all the food that is respired for life processes comes from photosynthesis. B. The energy released by oxidation in respiration is directly used for the life processes. C The carbon skeleton produced during respiration is used as precursors for biosynthesis of other molecules in cell. D. Plants, unlike animals, have no specialised organs for gaseous exchange but they have stomata and lenticels for this purpose. E. During oxidation within a cell, all the energy contained in, respiratory substrates is released free into the cell in a single step, which is trapped as chemical energy in the form of ATP.

A manufacturer produces three products A,B and C which he sells in market I and II. The annual sales are as shown below.(I) If the unit sale prices of A,B,C are Rs. 3, Rs. 2 and Rs. 4 respectively in market I and they are Rs. 4, Rs. 3 and Rs. 6 in market II. If the unit prices of A,B,C are Rs. 2.50, Rs. 1.50 and Rs. 3 respectively, then find the gross profit, using matrix algebra.

A manufacturer produces three products A,B and C which he sells in market I and II. The annual sales are as shown below.(I) If the unit sale prices of A,B,C are Rs. 3, Rs. 2 and Rs. 4 respectively in market I and they are Rs. 4, Rs. 3 and Rs. 6 in market II. Find the total revenue in each market using matrix algebra.