What is recombinant DNA ?

The first step in recombinant DNA technology is

What is recombination? Discuss the applications of recombinationfrom the point of viewof genetic engineering.

Mention the names of biological tools used in recombinant DNA technology.

The.... most commonly used in recombinant DNA technology are those that replicate in E. coli.

Rewrite the following paragraph by writting most appropriate words in the blank places. Insulin is a....... hormone produced by beta cells of islets of Langerhans of pancreas, insulin is essential for the control of blood sugar levels......... is a disease in which some people cannot make insulin themselves. Hakura et al, chemically synthesized DNA sequence of insulin for two chains....... and separately inserted into two....... vector. Insulin production by recombinant DNA technology is designed by Gilbert and Villa -Komaroff. The genes are inserted by the side of ..... gene of the plasmid. The recombinant plasmids were then separately transformed into E. coil host. The host produced penicillinase + pre-pro insulin . Insulin is later separated by ......... treatment.

Draw schematic representation of process of recombinant DNA technology and explain the given steps involved in it. Insertion of desired foreign gene into a cloning vector (vehicle DNA)

Genetic engineering is alternatively called as Recombinant DNA technology or Gene Cloning: Give scientific reason.

What is the use of DNA fingerprinting?

What is promoter in a transcription unit? Where is it located in DNA?

Explain the role genetic recombination in organic evolution.