Which among the following are steps of tissue culture?

Options

A. Removal of tissues from parent organism.
B. Inoculation of explant (excised tissue) in suitable culture medium.
C. Incubation of excised tissues in conducive environment.
D. All of the above

Answer

### Step-by-Step Solution for the Steps of Tissue Culture

1. **Removal of Tissue from Parent Organism**:
- The first step in tissue culture involves selecting and removing a small piece of tissue from the parent plant. This piece is referred to as the "explant." For example, if we are using a carrot, we might take a small section of the carrot tissue.

2. **Inoculation of Explant in Suitable Culture Medium**:
- After obtaining the explant, the next step is to place it in a nutrient-rich culture medium. This medium provides the necessary nutrients, hormones, and growth factors that support the growth and development of the plant tissue.

3. **Incubation of Explanted Tissues in a Conducive Environment**:
- The explant is then incubated in a controlled environment that is conducive for growth. This involves maintaining optimal temperature, humidity, and light conditions to encourage the tissue to grow and develop into new plant structures.

4. **Formation of Embryoids and Embryos**:
- As the tissue grows, it may differentiate and form structures such as embryoids or embryos. This is a crucial step as it leads to the development of new plantlets from the cultured tissue.

5. **Planting of Developed Plantlets**:
- Once the plantlets have developed sufficiently, they can be transferred to soil or another growing medium to continue their growth as independent plants.

### Summary of Steps:
- Removal of tissue from parent organism
- Inoculation of explant in suitable culture medium
- Incubation of explanted tissues in a conducive environment
- Formation of embryoids and embryos
- Planting of developed plantlets