Which one of the following techniques is used for the detection of proteins ?

Options

A. Northern blotting
B. Western blotting
C. Southern blotting
D. In-situ hybridization

Answer

To determine which technique is used for the detection of proteins, we will analyze the provided options step by step.

### Step-by-Step Solution:

1. **Understanding the Techniques**:
- **Northern Blotting**: This technique is primarily used for detecting specific RNA molecules. It involves the transfer of RNA from a gel to a membrane and subsequent hybridization with a labeled probe.
- **Southern Blotting**: This method is used to detect specific DNA sequences. Similar to Northern blotting, it transfers DNA from a gel to a membrane for hybridization with a probe.
- **Western Blotting**: This technique is specifically designed for the detection of proteins. It involves separating proteins by gel electrophoresis, transferring them to a membrane, and then detecting them using antibodies.
- **In-situ Hybridization**: This method is used to detect specific nucleic acid sequences (DNA or RNA) within fixed tissues or cells, not proteins.

2. **Identifying the Correct Technique**:
- Since the question asks for the detection of proteins, we can eliminate Northern blotting, Southern blotting, and in-situ hybridization, as they are not used for protein detection.
- Western blotting is the only technique listed that is specifically designed for detecting proteins.

3. **Conclusion**:
- The correct answer is **Western Blotting**, as it is the technique used for the detection of proteins.

### Final Answer:
**Western Blotting** is the technique used for the detection of proteins.