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Biuret test is used for the detection of...

Biuret test is used for the detection of:

A

sugar

B

proteins

C

fats

D

starch

Text Solution

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### Step-by-Step Solution: 1. **Understanding the Biuret Test**: The Biuret test is a chemical test used to detect the presence of proteins in a sample. It is based on the formation of a color change when proteins are present. 2. **Components of the Test**: The test involves an alkaline solution (usually sodium hydroxide) and a few drops of 1% copper sulfate (CuSO4) solution. 3. **Reaction Mechanism**: When the alkaline solution of the protein or polypeptide is treated with CuSO4, a reaction occurs. The Cu²⁺ ions from the copper sulfate interact with the peptide bonds present in the protein. 4. **Color Change Observation**: The presence of proteins is indicated by the formation of a violet color. This color change is due to the formation of a coordination complex between the Cu²⁺ ions and the nitrogen atoms in the peptide bonds. 5. **Conclusion**: The Biuret test is specifically used for the detection of proteins, as it relies on the presence of peptide bonds (C-O-N-H bonds) found in proteins and polypeptides. ### Final Answer: The Biuret test is used for the detection of **proteins**. ---

### Step-by-Step Solution: 1. **Understanding the Biuret Test**: The Biuret test is a chemical test used to detect the presence of proteins in a sample. It is based on the formation of a color change when proteins are present. 2. **Components of the Test**: The test involves an alkaline solution (usually sodium hydroxide) and a few drops of 1% copper sulfate (CuSO4) solution. 3. **Reaction Mechanism**: When the alkaline solution of the protein or polypeptide is treated with CuSO4, a reaction occurs. The Cu²⁺ ions from the copper sulfate interact with the peptide bonds present in the protein. ...
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