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What are bioreactors?...

What are bioreactors?

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Bioreactor used in biotechnology is generally used to process 100-1000 litre of material. It has a cylindrical metal container with a curved base to facilitate mixing of contents. The culture medium containing all the nutrients, salts, vitamins, hormones, etc. is added alongwith inoculum of transformed cells with recombinant DNA. A stirrer helps in mixing and optimum availability of nutrients to culture cells. Supply of oxygen is maintained if the cells function better under aerobic .conditions. Foam is kept under control. Gadgets are attached for knowing temperature and pH of the contents. Corrections are made when required. There is a sampling port where small volume of culture can be withdrawn to know the growth of cells and concentration of extractable product.
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BETTER CHOICE PUBLICATION-PRINCIPLES AND PROCESSES IN BIOTECHNOLOGY -SHORT ANSWER TYPE QUESTIONS
  1. What are restriction enzymes? What is their function?

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  2. Write down four common steps in sequence in recombinant technology.

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  3. What are bioreactors?

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  4. How can you distinguish between plasmid DNA and chromosomal DNA?

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  5. Distinguish between electroporation and micro-injection.

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  6. Discuss with your teacher and find mil how to distinguish between : E...

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  7. Write any two uses of gene cloning.

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  8. Write one use of PCR technique.

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  9. Define genetic engineering. Name one natural genetic engineer of plant...

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  10. What is embryonic stem cell technology? Name two animals which were pr...

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  11. Explain briefly: PCR

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  12. Define recombinant DNA technology. What is the function of restriction...

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  13. What is plasmid DNA? Why plasmids are suitable for use in genetic engi...

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  14. Why E. coli in used as competent host in rDNA technology?

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  15. How are restriction endonuclease enzymers are named? Write Example.

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  16. The Agrobacterium is considered as Natural genetic engineer of plants....

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  17. What are the basic requirements of PCR technique.

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  18. Write a short note on down streaming process.

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  19. Distinguish between Blunt ends and Sticky ends produced by restriction...

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  20. Which enzymes are used to isolate genetic material (DNA) in bacteria a...

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