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Explain briefly: PCR...

Explain briefly: PCR

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PCR is polymerase Chain Reaction. It is in vitro replication of DNA to form its multiple copies without a cell. It involves 3 steps-(i) Denaturation at `94^@C`. (ii) Anneating at `54^@C`, (iii) Extension at `72^@C`.
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BETTER CHOICE PUBLICATION-PRINCIPLES AND PROCESSES IN BIOTECHNOLOGY -SHORT ANSWER TYPE QUESTIONS
  1. Define genetic engineering. Name one natural genetic engineer of plant...

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  2. What is embryonic stem cell technology? Name two animals which were pr...

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  3. Explain briefly: PCR

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  4. Define recombinant DNA technology. What is the function of restriction...

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  5. What is plasmid DNA? Why plasmids are suitable for use in genetic engi...

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  6. Why E. coli in used as competent host in rDNA technology?

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  7. How are restriction endonuclease enzymers are named? Write Example.

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  8. The Agrobacterium is considered as Natural genetic engineer of plants....

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  9. What are the basic requirements of PCR technique.

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  10. Write a short note on down streaming process.

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  11. Distinguish between Blunt ends and Sticky ends produced by restriction...

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  12. Which enzymes are used to isolate genetic material (DNA) in bacteria a...

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  13. What do you mean by 'Golden rice' ?

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  14. What is Gene cloning? What are its advantages?

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  15. In plants, how is alien DNA introduced into host cell?

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  16. What is the importance of Plant tissue culture and Biotechnology?

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  17. What are the advantages and probable risks of Genetic engineering?

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  18. Describe briefly Downstream processing.

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  19. What is 'Golden rice'? How it can prevent child blindness?

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  20. Expand PCR.

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