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For selection of recombinants, insertion...

For selection of recombinants, insertional inactivation of antibiotic marker has been superseded by insertional inactivation of a marker gene coding for a chromogenic substrate. Give reasons.

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Selection of recombinants due to inactivation of antibiotics is cumbersome as it requires simultaneous plating of two plates having different antibiotics, Where as marker gene coding for chromogenic substrate differentiate recombinants from non-recombinants on basis of ability to produce colour in presence of chromogenic substrate.
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Assertion: Selection of recombinants due to inactivation of antibiotic is a cumbersome procedure. Reason: It requires simultaneous planting on two plates having different antibiotics.