Give the correct sequence of Hershey-Chase experiment :-

Options

A. Infection, centrifugation, blending
B. Blending, infection, centrifugation
C. Infection, blending, centrifugation
D. Centrifugation, infection, blending

Answer

To understand the correct sequence of the Hershey-Chase experiment, let's break down the steps involved in the experiment:

### Step-by-Step Solution:

1. **Infection**: The first step involves allowing the bacteriophages (viruses that infect bacteria) to infect the E. coli bacteria. In this experiment, two types of bacteriophages are used: one labeled with radioactive phosphorus (which labels DNA) and another labeled with radioactive sulfur (which labels proteins). This step is crucial as it initiates the infection process where the bacteriophages inject their genetic material into the bacterial cells.

2. **Blending**: After the infection period, the next step is blending. This involves using a blender to agitate the mixture of infected bacteria and bacteriophages. The purpose of this step is to separate the viral coats from the bacteria, ensuring that only the material that has entered the bacteria remains.

3. **Centrifugation**: The final step is centrifugation. In this step, the blended mixture is spun in a centrifuge. This process separates the heavier bacterial cells from the lighter viral particles. The bacteria, which contain the injected genetic material, will form a pellet at the bottom of the centrifuge tube, while the viral particles remain in the supernatant.

### Conclusion:
The correct sequence of the Hershey-Chase experiment is:
1. Infection
2. Blending
3. Centrifugation