Enzyme used for obtaining protoplast from isolated single cell of plant are :-

Options

A. Cellulose and lysozyme
B. Cellulase and pectinase
C. Protease and lipase
D. Cellulase and Dnase

Answer

To answer the question regarding the enzymes used for obtaining protoplasts from isolated single cells of plants, we can follow these steps:

### Step-by-Step Solution:

1. **Understand Protoplasts**: Protoplasts are plant cells that have had their cell wall removed. They consist of the plasma membrane and the contents of the cell, including the cytoplasm and organelles.

2. **Identify the Need for Enzymes**: To isolate protoplasts, the cell wall of the plant cell must be digested. This is where specific enzymes come into play.

3. **Recognize the Composition of Cell Walls**: Plant cell walls are primarily made up of cellulose, hemicellulose, and pectin. Therefore, enzymes that can break down these components are required.

4. **Select Appropriate Enzymes**:
- **Cellulase**: This enzyme breaks down cellulose, which is a major component of the plant cell wall.
- **Pectinase**: This enzyme breaks down pectin, another significant component of the cell wall.

5. **Evaluate Other Options**:
- **Lysozyme**: While it digests bacterial cell walls, it is not effective for plant cell walls.
- **Protease**: This enzyme breaks down proteins, which is not relevant for cell wall digestion.
- **Lipase**: This enzyme digests fats and is not involved in cell wall breakdown.
- **DNase**: This enzyme breaks down DNA, which is also not relevant for isolating protoplasts.

6. **Conclusion**: The correct enzymes for obtaining protoplasts from isolated single cells of plants are **cellulase and pectinase**.

### Final Answer:
The enzymes used for obtaining protoplasts from isolated single cells of plants are **cellulase and pectinase**.

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