Find out incorrect match:

Options

A. PKU - autosomal recessive
B. `alpha-1` antitrypsin - used to treat emhysema
C. ELISA - it is based on the antibody reaction only
D. alpha - lactalbumin protein - rosie

Answer

To find the incorrect match from the given options, we will analyze each match one by one.

### Step-by-Step Solution:

1. **PKU (Phenylketonuria)**:
- PKU stands for Phenylketonuria, which is indeed an autosomal recessive disorder.
- It is caused by a mutation in both alleles of the gene for phenylalanine hydroxylase located on chromosome 12.
- **Conclusion**: This match is correct.

2. **Alpha 1 Antitrypsin**:
- Alpha 1 antitrypsin is a protein that protects the lungs from damage.
- A deficiency in this protein can lead to lung diseases such as emphysema.
- It is produced in transgenic sheep for therapeutic purposes.
- **Conclusion**: This match is correct.

3. **ELISA (Enzyme-Linked Immunosorbent Assay)**:
- ELISA is a plate-based assay technique designed for detecting and quantifying not only antibodies but also proteins, peptides, and hormones.
- The statement that it is based solely on antibody reactions is incorrect.
- **Conclusion**: This match is incorrect.

4. **Rosy Cow and Alpha-Lactalbumin**:
- The rosy cow is a transgenic cow that produces human alpha-lactalbumin protein.
- Alpha-lactalbumin is a major milk protein involved in lactose synthesis.
- **Conclusion**: This match is correct.

### Final Conclusion:
The incorrect match is the third option regarding ELISA, as it is not solely based on antibody reactions.

---