Which one of the following technique is not used to produce transgenic animals :-

Options

A. Microinjection
B. Micropropagation
C. Animals cell culture
D. In-vitro fertilization

Answer

To determine which technique is not used to produce transgenic animals, we need to analyze the given options: microinjection, micropropagation, animal cell culture, and in vitro fertilization.

**Step 1: Understand the Techniques**
- **Microinjection**: This technique involves directly injecting DNA into the nucleus of a fertilized egg. It is commonly used to create transgenic animals by introducing new genes.

- **Micropropagation**: This is a technique primarily used for plants. It involves the vegetative propagation of plants through tissue culture methods. It is not applicable to animals.

- **Animal Cell Culture**: This technique involves growing animal cells in a controlled environment outside of their natural habitat. It can be used in the development of transgenic animals.

- **In Vitro Fertilization (IVF)**: This is a process where fertilization occurs outside the body, and it can be used to create embryos that may be genetically modified before implantation in a surrogate mother.

**Step 2: Identify the Correct Answer**
From the analysis, we can see that:
- Microinjection, animal cell culture, and in vitro fertilization are all techniques used to produce transgenic animals.
- Micropropagation, however, is specifically a plant-based technique and is not used for animals.

Thus, the technique that is not used to produce transgenic animals is **micropropagation**.

**Final Answer: Micropropagation**

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