Find incorrect statement with respect to genetically engineered insulin :-

Options

A. C' peptide is not present in mature insulin
B. A' and 'B' peptide chain produced separately by Eli-Lilly
C. Human insulin is produced by plasmid of E.coli
D. Chain A and B, that are linked by H-bond (hydrogen bond)

Answer

To find the incorrect statement with respect to genetically engineered insulin, let's analyze the statements provided:

1. **C-peptide is not present in mature insulin.**
- This statement is correct. C-peptide is indeed removed during the maturation of insulin.

2. **A and B peptide chains are produced separately by alleles.**
- This statement is also correct. The A and B chains of insulin are produced separately through recombinant DNA technology.

3. **Human insulin is produced by plasmid of E. coli.**
- This statement is correct. The plasmid of E. coli is used to produce the insulin chains.

4. **Chains A and B are linked by a hydrogen bond.**
- This statement is incorrect. Chains A and B of insulin are linked by disulfide bonds, not hydrogen bonds.

### Conclusion:
The incorrect statement is: **Chains A and B are linked by a hydrogen bond.**

### Step-by-Step Solution:
1. **Identify the statements regarding genetically engineered insulin.**
- Review each statement to understand its validity.

2. **Evaluate the first statement about C-peptide.**
- Confirm that C-peptide is removed during the maturation of insulin, making this statement correct.

3. **Evaluate the second statement about A and B chains.**
- Acknowledge that A and B chains are produced separately, which is accurate.

4. **Evaluate the third statement regarding E. coli.**
- Recognize that E. coli is indeed used to produce human insulin, confirming this statement is correct.

5. **Evaluate the fourth statement about the bonding between chains.**
- Identify that the correct type of bond linking chains A and B is disulfide bonds, not hydrogen bonds, making this statement incorrect.

6. **Conclude which statement is incorrect.**
- Based on the evaluations, conclude that the fourth statement is the incorrect one.