Callase enzyme which dissolved callose of pollen tetrads to separate four pollens is provided by

Options

A. Pollen grains
B. Middle layer
C. Tapetum
D. Endothecium

Answer

### Step-by-Step Solution:

1. **Understanding the Context**: The question is about the callase enzyme, which is responsible for dissolving callose in pollen tetrads, allowing the separation of individual pollen grains.

2. **Identifying the Source of Callase**: According to the information provided, the callase enzyme is produced by a specific layer within the anther of flowering plants.

3. **Layers of the Anther**: The anther consists of several layers:
- **Epidermis**: The outermost layer.
- **Endothecium**: The layer beneath the epidermis.
- **Middle Layer**: A layer that provides some support.
- **Tapetum**: The innermost layer that is crucial for nourishment.

4. **Role of the Tapetum**: The tapetum is described as a specialized nutritive tissue that provides nourishment to the developing pollen grains. Importantly, it also produces the callase enzyme.

5. **Function of Callase**: The callase enzyme acts on the callose, a polysaccharide that forms the walls between pollen grains in a tetrad. By dissolving this callose, callase facilitates the separation of the four pollen grains.

6. **Conclusion**: Therefore, the correct answer to the question is that the callase enzyme, which dissolves callose in pollen tetrads, is provided by the **tapetum** layer of the anther.

### Final Answer:
The callase enzyme which dissolves callose of pollen tetrads to separate four pollens is provided by the **tapetum**.

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