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T(m) melting temperature) value of DNA i...

`T_(m)` melting temperature) value of DNA is high when if contains

A

`A+TgtG+`

B

`G+CgtA+T`

C

`A+T=G+C`

D

`A+G=T+C`

Text Solution

AI Generated Solution

The correct Answer is:
To determine when the melting temperature (T_m) of DNA is high, we need to consider the composition of the DNA strands, specifically the types of nitrogenous bases they contain. Here’s a step-by-step breakdown of the reasoning: ### Step 1: Understand DNA Structure DNA is composed of two strands that are held together by pairs of nitrogenous bases. There are two types of nitrogenous bases: purines and pyrimidines. ### Step 2: Identify the Nitrogenous Bases - **Purines**: Adenine (A) and Guanine (G) - **Pyrimidines**: Cytosine (C) and Thymine (T) ### Step 3: Base Pairing The bases pair as follows: - Adenine (A) pairs with Thymine (T) using **two hydrogen bonds**. - Guanine (G) pairs with Cytosine (C) using **three hydrogen bonds**. ### Step 4: Impact on Melting Temperature The melting temperature (T_m) of DNA is influenced by the number of hydrogen bonds between the base pairs: - The more hydrogen bonds present, the higher the T_m. - Since G-C pairs have three hydrogen bonds, they are more stable than A-T pairs, which only have two hydrogen bonds. ### Step 5: Conclusion Therefore, the T_m of DNA is higher when the DNA contains a higher proportion of Guanine (G) and Cytosine (C) bases compared to Adenine (A) and Thymine (T) bases. This means that DNA with a higher G-C content will have a higher melting temperature. ### Final Answer The T_m value of DNA is high when it contains a higher proportion of Guanine (G) and Cytosine (C) bases compared to Adenine (A) and Thymine (T) bases. ---
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