Which techniques is used by Mendel for hybridisation

Options

A. Emasculation
B. Bagging
C. Protoplast fusion
D. 1 & both

Answer

**Step-by-Step Solution:**

1. **Understanding Hybridization in Mendel's Experiments:**
Mendel's experiments focused on the principles of inheritance using pea plants. He aimed to understand how traits are passed from one generation to the next through hybridization.

2. **Emasculation:**
The first technique Mendel used was emasculation. This involves the removal of the anthers from the flower before they mature and release pollen. By doing this, Mendel prevented self-pollination, ensuring that the flower would not fertilize itself.

3. **Bagging:**
After emasculation, the next step was bagging. The emasculated flower was covered with a small paper or polythene bag. This prevented any contamination from other pollen sources, ensuring that any fertilization that occurred would be from pollen that Mendel introduced himself.

4. **Conclusion:**
The two techniques used by Mendel for hybridization were emasculation and bagging. Protoplast fusion, which is a modern technique, was not used by Mendel in his experiments.

5. **Final Answer:**
The correct answer to the question is that Mendel used the techniques of emasculation and bagging for hybridization.

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