The DNA dependent DNA polymerase catalyse polymerisation in -

Options

A. `3' to 5'` direction
B. `5' to 3'` direction
C. Depend on the nature of template strand
D. Both (1) & (2)

Answer

**Step-by-Step Solution:**

1. **Understanding DNA Polymerase**: DNA-dependent DNA polymerase is an enzyme that synthesizes DNA molecules from deoxyribonucleotides, the building blocks of DNA.

2. **Direction of Polymerization**: DNA polymerase synthesizes DNA in a specific direction. It requires a 3' hydroxyl group to initiate the addition of new nucleotides.

3. **Template Strand Orientation**: The enzyme moves along the template strand, which runs in the 3' to 5' direction. This is crucial because the new strand is synthesized in the opposite direction.

4. **Synthesis of the New Strand**: As the DNA polymerase moves along the template strand, it synthesizes the new DNA strand in the 5' to 3' direction. This means that nucleotides are added to the 3' end of the growing strand.

5. **Conclusion**: Based on the above understanding, the correct answer to the question is that DNA-dependent DNA polymerase catalyzes polymerization in the 5' to 3' direction.

**Final Answer**: The DNA-dependent DNA polymerase catalyzes polymerization in the **5' to 3' direction**.

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