The technique of transferring DNA fargment separated on agarose gel to a synthetic membrane such as nitrocellulose is known as

Options

A. Nothern blotting
B. Southern bolltting
C. Western boltting
D. Dot boltting

Answer

To answer the question regarding the technique of transferring DNA fragments separated on agarose gel to a synthetic membrane such as nitrocellulose, we can follow these steps:

### Step-by-Step Solution:

1. **Understanding the Techniques**:
- We need to identify the techniques mentioned in the options: Northern blotting, Southern blotting, Western blotting, and Dot blotting.

2. **Northern Blotting**:
- This technique is specifically used to detect RNA (mRNA) sequences. It involves transferring RNA from an agarose gel to a membrane, but it does not pertain to DNA.

3. **Western Blotting**:
- Western blotting is used for the detection of proteins. It involves transferring proteins from a gel to a membrane, typically nitrocellulose or PVDF, but it is not related to DNA.

4. **Dot Blotting**:
- Dot blotting is a simpler technique used for the identification of proteins or nucleic acids, but it does not involve the transfer of fragments from an agarose gel.

5. **Southern Blotting**:
- Southern blotting is the technique that specifically refers to the transfer of DNA fragments from an agarose gel to a membrane (such as nitrocellulose). This technique allows for the detection of specific DNA sequences.

6. **Conclusion**:
- Based on the definitions and uses of the techniques, the correct answer to the question is **Southern blotting**.

### Final Answer:
The technique of transferring DNA fragments separated on agarose gel to a synthetic membrane such as nitrocellulose is known as **Southern blotting**.

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