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The transfer of protein from electophore...

The transfer of protein from electophoretic gel to nitrocellulose membrane is known as

A

transferase

B

northern blotting

C

western boltting

D

southern blotting

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### Step-by-Step Solution: 1. **Understanding the Question**: The question asks about the process of transferring proteins from an electrophoretic gel to a nitrocellulose membrane. 2. **Identifying the Techniques**: There are several techniques used for transferring biological molecules from gels to membranes, including: - Southern blotting (for DNA) - Northern blotting (for RNA) - Western blotting (for proteins) 3. **Focusing on the Correct Technique**: Since the question specifically mentions proteins, we need to identify which technique is used for protein transfer. - Southern blotting is not applicable as it deals with DNA. - Northern blotting is also not applicable as it deals with RNA. 4. **Confirming the Correct Answer**: The technique used for transferring proteins from an electrophoretic gel to a nitrocellulose membrane is known as **Western blotting**. This method involves the use of antibodies to detect specific proteins after they have been separated by gel electrophoresis. 5. **Conclusion**: The correct answer to the question is **Western blotting**.
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ALLEN-GENETICS & MOLECULAR BASIS OF INHERITANCE AND MUTATION-EXERCISE-I (CONCEPTUAL QUESTIONS)
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  7. Which one of the following pairs of terms/names are the same?

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  8. What is the first step in the southem Blot technique?

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  9. Which step does not involve in DNA finger printing

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  10. The technique of transferring DNA fargment separated on agarose gel to...

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  11. Western blotting is used for the identification of :-

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  12. Recent techniques used for separating fragments of DNA is

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  13. The approximate number of genes contained in the genome of Kalpana Cha...

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  16. The method of DNA fingerprinting involves the use of :

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  17. Which of the following is not associated with HGP-

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  18. In density gradient centrifugation, the bulk DNA forms while satelite ...

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  19. Which step is not correct in DNA finger printing-

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  20. DNA fingerprinting method is very useful for

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