Safranin and fast green stain which tissue respectively

Options

A. Meristem and parenchyma
B. Xylem and phloem
C. Parenchyma and collenchyma
D. All of the above

Answer

### Step-by-Step Solution:

1. **Understanding the Stains**: Safranin and Fast Green are dyes used in histology to stain plant tissues. Each dye has a specific affinity for certain types of tissues based on their composition.

2. **Identifying Safranin's Function**: Safranin is primarily used to stain tissues that contain lignin. Lignin is a complex organic polymer found in the cell walls of many plants, providing rigidity and resistance to decay. The tissues that typically contain lignin include xylem and sclerenchyma.

3. **Identifying Fast Green's Function**: Fast Green is a dye that stains cellulose-rich tissues, such as phloem. It provides a green or blue color to these tissues, while it does not stain lignin-rich tissues as effectively.

4. **Analyzing the Options**:
- **Option 1: Meristem and Parenchyma**: Parenchyma does not contain lignin, so it would not be stained by Safranin. This option is incorrect.
- **Option 2: Xylem and Phloem**: Xylem contains lignin and will be stained by Safranin, while phloem, which contains cellulose, will be stained by Fast Green. This option is correct.
- **Option 3: Parenchyma and Collenchyma**: Both of these tissues consist primarily of cellulose and do not contain lignin, so they would not be stained by Safranin. This option is incorrect.
- **Option 4: All of the Above**: Since not all options are correct, this option is also incorrect.

5. **Conclusion**: The correct answer is **Option 2: Xylem and Phloem**. Safranin stains xylem due to its lignin content, while Fast Green stains phloem.