Taq-polymerase which is used for amplification of DNA related with

Options

A. Hybridoma technique
B. PCR -technique
C. Gene cloning
D. r-DNA technology

Answer

**Step-by-Step Solution:**

1. **Understanding Taq Polymerase**: Taq polymerase is a type of DNA polymerase that is heat-stable. This characteristic is crucial for its function in DNA amplification processes.

2. **Source of Taq Polymerase**: Taq polymerase is derived from the bacterium *Thermus aquaticus*, which is a thermophilic (heat-loving) bacterium. This allows the enzyme to withstand the high temperatures used in DNA amplification.

3. **Role in PCR**: The primary application of Taq polymerase is in the Polymerase Chain Reaction (PCR) technique. PCR is a widely used method for amplifying specific DNA sequences, making millions of copies of a particular DNA segment.

4. **Process of PCR**: During PCR, the reaction involves repeated cycles of denaturation (heating the DNA to separate strands), annealing (cooling to allow primers to bind), and extension (using Taq polymerase to synthesize new DNA strands). The heat-stability of Taq polymerase is essential during the denaturation step, where the temperature is significantly increased.

5. **Conclusion**: Given the specific function and properties of Taq polymerase, it is directly related to the PCR technique for DNA amplification. Therefore, the correct answer to the question is that Taq polymerase is related to the PCR technique.

**Final Answer**: Taq-polymerase is used for amplification of DNA related to the PCR technique.

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