Which technique is used to check the progression of restriction enzyme digestion

Options

A. PCR
B. Gel electrophorosis
C. Southern Blotting
D. Staining

Answer

To answer the question "Which technique is used to check the progression of restriction enzyme digestion?", we can follow these steps:

### Step-by-Step Solution:

1. **Understanding Restriction Enzymes**:
- Restriction enzymes, also known as restriction endonucleases, are proteins that can cut DNA at specific sequences. This process is known as restriction digestion.

2. **Purpose of Checking Progression**:
- After performing a restriction digestion, it is important to verify that the DNA has been cut at the expected sites and to analyze the resulting fragments.

3. **Techniques for Analyzing DNA Fragments**:
- There are several techniques used to analyze DNA, but the most relevant for checking the progression of restriction enzyme digestion is gel electrophoresis.

4. **Gel Electrophoresis**:
- Gel electrophoresis is a technique that separates DNA fragments based on their size. When DNA is loaded into a gel matrix and an electric current is applied, smaller fragments move faster and further through the gel than larger ones.

5. **Interpreting Results**:
- After running the gel, the DNA fragments can be visualized (often using a DNA stain) to determine their sizes. By comparing the positions of the bands on the gel with a DNA ladder (a mixture of known DNA sizes), one can confirm whether the restriction digestion has occurred as expected.

6. **Conclusion**:
- Therefore, the technique used to check the progression of restriction enzyme digestion is **gel electrophoresis**.

### Final Answer:
The technique used to check the progression of restriction enzyme digestion is **gel electrophoresis**.

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