In get electrophorosis, at which end of the gel the sample is loaded ?

Options

A. In the wells
B. Towards positive electrode
C. Towards negative electrode
D. 1 & 3 both

Answer

**Step-by-Step Solution:**

1. **Understanding Gel Electrophoresis**: Gel electrophoresis is a technique used to separate macromolecules like DNA, RNA, or proteins based on their size and charge.

2. **Identifying the Electrodes**: In gel electrophoresis, there are two electrodes:
- The **positive terminal** (anode)
- The **negative terminal** (cathode)

3. **Charge of the Samples**: DNA and RNA are both negatively charged due to their phosphate backbone. This means that when an electric current is applied, they will move towards the positive terminal.

4. **Loading the Sample**: Since the samples (DNA or RNA) are negatively charged, they are loaded at the **negative terminal** (cathode) of the gel. This is done to ensure that when the electric current is applied, the samples will migrate towards the positive terminal.

5. **Conclusion**: Therefore, in gel electrophoresis, the sample is loaded at the **negative end** of the gel.

**Final Answer**: The sample is loaded at the negative end (cathode) of the gel.

---