Which of the following step is not involved in basic steps in genetically modifying an organism

Options

A. Identification of DNA with desirable gene
B. Introduction of the identified DNA into the host
C. Amplification of DNA by using PCR
D. Maintenance of introduced DNA in the host and transfer of the DNA to its progeny

Answer

**Step-by-Step Solution:**

1. **Understand the Question**: The question asks which step is not involved in the basic steps of genetically modifying an organism (GMO).

2. **Identify the Steps Provided**: The steps given are:
- Identification of DNA with a desirable gene.
- Introduction of the identified DNA into the host.
- Amplification of DNA by using PCR.
- Maintenance of introduced DNA in the host and transfer of the DNA to its progeny.

3. **Analyze Each Step**:
- **Step 1**: Identification of DNA with a desirable gene is essential for determining which gene will be introduced into the organism.
- **Step 2**: Introduction of the identified DNA into the host is a critical step in the genetic modification process, often done using vectors.
- **Step 3**: Amplification of DNA by using PCR (Polymerase Chain Reaction) is a technique used to make multiple copies of a specific DNA segment. While important in molecular biology, it is not a direct step in the process of creating a GMO.
- **Step 4**: Maintenance of introduced DNA in the host and transfer of the DNA to its progeny is crucial for ensuring that the genetic modification is stable and passed on to future generations.

4. **Determine the Incorrect Step**: Upon analyzing the steps, it is clear that the third step (Amplification of DNA by using PCR) is not directly involved in the basic steps of genetically modifying an organism.

5. **Conclusion**: The correct answer is that the third option, "Amplification of DNA by using PCR," is not involved in the basic steps of genetically modifying an organism.

**Final Answer**: The step that is not involved in the basic steps of genetically modifying an organism is "Amplification of DNA by using PCR."

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