Which of the following enzyme will get inactivated in insertional inactivation

Options

A. Transacetylase
B. Permease
C. `beta-` galactosidase
D. Taq-polymerase

Answer

To solve the question "Which of the following enzyme will get inactivated in insertional inactivation?" we will follow these steps:

### Step 1: Understand Insertional Inactivation
Insertional inactivation is a process that occurs during recombinant DNA technology. It involves the insertion of a DNA fragment into a gene, which can disrupt the function of that gene.

**Hint:** Remember that insertional inactivation specifically refers to the disruption of a gene's function due to the insertion of foreign DNA.

### Step 2: Identify the Enzymes Listed
The options provided are:
1. Trans acetylase
2. Permease
3. Beta galactosidase
4. Taq polymerase

**Hint:** Familiarize yourself with the functions of these enzymes to understand which one is affected by insertional inactivation.

### Step 3: Analyze the Function of Each Enzyme
- **Trans acetylase:** Involved in the acetylation of compounds, not typically associated with insertional inactivation.
- **Permease:** A transport protein that facilitates the movement of molecules across membranes, not directly linked to insertional inactivation.
- **Beta galactosidase:** An enzyme that breaks down lactose into glucose and galactose. It is often used as a reporter gene in recombinant DNA technology.
- **Taq polymerase:** A DNA polymerase used in PCR, not affected by insertional inactivation.

**Hint:** Focus on the enzyme that is commonly used in cloning and recombinant DNA techniques.

### Step 4: Determine Which Enzyme Gets Inactivated
In the context of insertional inactivation, when a foreign DNA is inserted into the gene coding for beta galactosidase, it disrupts the enzyme's function. Therefore, beta galactosidase is the enzyme that gets inactivated.

**Hint:** Recall that beta galactosidase is often used as a marker in cloning experiments, making it a prime candidate for inactivation through insertional inactivation.

### Step 5: Conclusion
The enzyme that gets inactivated in insertional inactivation is **beta galactosidase**.

**Final Answer:** Beta galactosidase