The prerequisties for biotechnological production of antibiotics is

Options

A. To search an antibiotic producing microorganism
B. To isolate the antibiotic gene
C. To join antibiotic gene with E.coli plasmid
D. All of the above

Answer

To answer the question regarding the prerequisites for biotechnological production of antibiotics, we can break down the process into several key steps:

### Step-by-Step Solution:

1. **Identify Antibiotic-Producing Microorganisms**:
- The first step in the biotechnological production of antibiotics is to search for microorganisms that are known to produce antibiotics. These can be bacteria (like *Streptomyces*) or fungi (like *Penicillium*).

2. **Isolate the Antibiotic Gene**:
- Once the antibiotic-producing microorganism is identified, the next step is to isolate the specific gene responsible for antibiotic production. This involves using molecular biology techniques to extract and purify the DNA that encodes the antibiotic.

3. **Join Antibiotic Gene with E. coli Plasmid**:
- After isolating the antibiotic gene, it needs to be inserted into a plasmid vector, such as one derived from *E. coli*. This step is crucial for the gene to be expressed in a host organism, allowing for the mass production of the antibiotic.

4. **Conclusion**:
- All of the above steps are essential prerequisites for the biotechnological production of antibiotics. Therefore, the correct answer to the question is "all of the above."

### Final Answer:
The prerequisites for biotechnological production of antibiotics include searching for antibiotic-producing microorganisms, isolating the antibiotic gene, and joining the antibiotic gene with an E. coli plasmid. Thus, the correct answer is **all of the above**.

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