Which one is used as a vector for gene transfer clonning gene ?

Options

A. Salmonella typhimurium DNA
B. Ti plasmid
C. Antibiotic resistance Amp' and Ter' loci
D. Ori minus pBR 322

Answer

To answer the question "Which one is used as a vector for gene transfer cloning gene?", we need to analyze the options provided and identify the correct vector used in genetic engineering.

### Step-by-Step Solution:

1. **Understanding the Concept of Vectors**:
- Vectors are DNA molecules used to deliver genetic material into cells. They are essential tools in genetic engineering for cloning genes.

2. **Evaluating the Options**:
- The options given are:
- Salmonella Typhi
- Morium
- DNA
- Ti-plasmid

3. **Analyzing Salmonella Typhi**:
- Salmonella Typhi is a pathogenic bacterium that causes typhoid fever. It is not used as a vector for gene cloning due to its pathogenic nature.

4. **Analyzing Morium**:
- There is no well-known vector or organism called "Morium" in the context of gene cloning. This option is likely incorrect.

5. **Analyzing DNA**:
- While DNA is the genetic material, it is not a vector by itself. Vectors are specific types of DNA molecules that can replicate independently within a host.

6. **Analyzing Ti-plasmid**:
- Ti-plasmid (Tumor-inducing plasmid) is derived from Agrobacterium tumefaciens. It is widely used as a vector in plant genetic engineering because it can transfer genes into plant cells effectively.

7. **Conclusion**:
- The correct answer is **Ti-plasmid** as it is specifically designed for gene transfer and cloning in plant cells.

### Final Answer:
The vector used for gene transfer and cloning is **Ti-plasmid**.

---