Which one of following is method of gene silencing

Options

A. tRNA
B. rRNA
C. RNAi
D. mRNA

Answer

To answer the question "Which one of the following is a method of gene silencing?", we will analyze each option provided:

1. **Understanding Gene Silencing**:
- Gene silencing is a process that suppresses the expression of specific genes. This can be achieved through various methods, particularly using RNA technology.

2. **Analyzing the Options**:
- **tRNA (Transfer RNA)**: This type of RNA is responsible for transporting amino acids to the ribosome during protein synthesis. It does not play a role in gene silencing.
- **rRNA (Ribosomal RNA)**: This RNA component is part of the ribosome and is essential for protein synthesis. Like tRNA, it does not contribute to gene silencing.
- **mRNA (Messenger RNA)**: This RNA carries genetic information from DNA to the ribosome for protein synthesis. It is not involved in gene silencing.
- **RNAi (RNA interference)**: This is a specific method of gene silencing. It involves the introduction of double-stranded RNA that is complementary to the target mRNA, leading to the degradation of that mRNA and preventing protein synthesis.

3. **Conclusion**:
- Among the options provided, RNAi is the only method that specifically functions in gene silencing. Therefore, the correct answer is **C. RNAi**.

### Step-by-Step Solution:
1. Define gene silencing and its purpose.
2. Examine each RNA type (tRNA, rRNA, mRNA) and their functions.
3. Identify RNAi as a method of gene silencing.
4. Conclude that RNAi is the correct answer.