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Find out correct recognisation sequence ...

Find out correct recognisation sequence of following restriction endonuclease enzyme

A

`{:(Bam HI,Eco RI),("GGATCC","GAATTC"),("CCTAGG","CTTAAG"):}`

B

`{:(Bam HI,Eco RI),("GAATCAA","TTGCAAC"),("CTTAGTT","AACGTTG"):}`

C

`{:(Bam HI,Eco RI),("GCATGG","AGCTCC"),("CGTACC","TCGAGG"):}`

D

`{:(Bam HI,Eco RI),("GACTAA","GCCTTA"),("CTGATT","CGGAAT"):}`

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AI Generated Solution

The correct Answer is:
To solve the question regarding the correct recognition sequences of the restriction endonuclease enzymes BMH1 and EcoRI, we can follow these steps: ### Step-by-Step Solution: 1. **Understand the Concept of Restriction Endonucleases**: - Restriction endonucleases are enzymes that cut DNA at specific recognition sequences. These sequences are often palindromic, meaning they read the same in the 5' to 3' direction on both strands of the DNA. 2. **Identify the Enzymes**: - The enzymes mentioned are BMH1 (from Bacillus amyloliquefaciens) and EcoRI (from Escherichia coli). 3. **Determine the Recognition Sequence for EcoRI**: - EcoRI recognizes the palindromic sequence: **GAATTC**. - When read from the opposite strand, it also reads as **GAATTC**. 4. **Determine the Recognition Sequence for BMH1**: - BMH1 recognizes the palindromic sequence: **GAGACC**. - This means that when read from the opposite strand, it also reads as **GAGACC**. 5. **Compare the Given Options**: - Now, we need to check the provided options to see which one correctly lists the recognition sequences for both enzymes. 6. **Select the Correct Option**: - After comparing, we find that the correct option is the one that lists EcoRI as **GAATTC** and BMH1 as **GAGACC**. ### Final Answer: The correct recognition sequences for the restriction endonucleases are: - EcoRI: GAATTC - BMH1: GAGACC The correct option is **Option 1**. ---
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