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Assertion: The stickiness of ends produc...

Assertion: The stickiness of ends produced by restriction endonuclease faciliates the action of DNA polymerase
Reason :It helps in joining the two DNA strands

A

If both Assertion & Reason are True & the Reason is a correct explanation of the Assertion

B

It both Assertion & Reason are True but Reason is not a correct explanation of the Assetion

C

If Assertion is True but the Reason is False

D

If both Assertion & Reason are false

Text Solution

AI Generated Solution

The correct Answer is:
To solve the assertion-reason question, let's break down the components step by step. ### Step 1: Understand the Assertion The assertion states: "The stickiness of ends produced by restriction endonuclease facilitates the action of DNA polymerase." - **Explanation**: Restriction endonucleases, such as EcoRI, cut DNA at specific sequences, creating "sticky ends." These sticky ends are overhanging sequences of DNA that can easily anneal to complementary sequences. This stickiness is crucial for the action of DNA polymerase, as it allows the enzyme to effectively bind and extend the DNA strands. ### Step 2: Understand the Reason The reason states: "It helps in joining the two DNA strands." - **Explanation**: While the sticky ends do facilitate the binding of DNA strands, the role of DNA polymerase is not to join the two strands directly. Instead, DNA polymerase synthesizes new DNA by adding nucleotides to a growing strand. The actual joining of the two DNA strands is typically done by another enzyme called DNA ligase. ### Step 3: Evaluate the Truth of Each Statement - **Assertion**: True. The stickiness of ends indeed facilitates the action of DNA polymerase. - **Reason**: False. The reason provided does not accurately describe the role of DNA polymerase. ### Step 4: Determine the Correct Option Based on the evaluations: - The assertion is true, but the reason is false. Therefore, the correct option is: **Option C: Assertion is true but reason is false.** ### Final Answer The correct answer is Option C: Assertion is true but reason is false. ---
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ALLEN-BIOTECHNOLOGY AND MICROBES IN HUMAN WELFARE-Exercise IV
  1. Assertion: The name of any restriction endonuclease ends with a Roman ...

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  2. Assertion: Restriction endonucleases produces overhanging stretches ca...

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  3. Assertion: The stickiness of ends produced by restriction endonuclease...

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  4. Assertion (A) : Restriction endonucleases recognise short pallindromic...

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  5. Assertion: The recombinant DNA molecule cannot be created, unless one ...

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  6. Assertion: The DNA fragments can be separated by a technique known as ...

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  7. Assertion: One can see bright orange coloured band of DNA in a ethidiu...

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  8. Assertion: In Gel electrophorosis, ''the smalller the fragment size, t...

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  9. Assertion: Presence of restriction sites of many enzymes within the ve...

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  10. Assertion: A good vector in that whose ori support high copy number ...

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  11. Assertion : Newer Antibiotics are required to be prosuced Regula...

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  12. Assertion: The inactivation of any vector gene due to insertion of des...

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  13. Assertion: Recombinant colones will produce blue colour in the medium ...

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  14. Assertion: Ti-plasmids & retrovirus are used as vectors in gene transf...

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  15. Assertion: DNA cannot pass through cell membranes Reason :DNA is a h...

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  16. Assertion: Bacterial cells first be made competent by monovalent catio...

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  17. Assertion: In biolistics method, cells are bombarded with high velocit...

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  18. Which technique is used to check the progression of restriction enzyme...

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  19. Assertion: A thermostable DNA polymerase in used in PCR for repeated a...

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  20. Recombinant DNA bearing ampicillin resistance gene is passed in E. col...

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