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Assertion: In Gel electrophorosis, ''the...

Assertion: In Gel electrophorosis, ''the smalller the fragment size, the farther it moves''
Reason :The separation of DNA segments by cutting out the gel is known as elution

A

If both Assertion & Reason are True & the Reason is a correct explanation of the Assertion

B

It both Assertion & Reason are True but Reason is not a correct explanation of the Assetion

C

If Assertion is True but the Reason is False

D

If both Assertion & Reason are false

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The correct Answer is:
### Step-by-Step Solution: 1. **Understanding the Assertion**: The assertion states that "in gel electrophoresis, the smaller the fragment size, the farther it moves." This is true because smaller DNA fragments can navigate through the pores of the gel more easily than larger fragments, allowing them to travel further when an electric current is applied. 2. **Understanding the Reason**: The reason provided is that "the separation of DNA segments by cutting out the gel is known as elution." This statement is also true. Elution refers to the process of extracting a substance from a solid by washing with a solvent, in this case, cutting out the gel to obtain specific DNA segments. 3. **Evaluating the Relationship**: While both the assertion and reason are true, the reason does not explain why smaller fragments move farther in gel electrophoresis. The reason describes a process (elution) that occurs after the electrophoresis has taken place, rather than explaining the mechanism behind the assertion. 4. **Selecting the Correct Option**: Since both the assertion and reason are true, but the reason does not provide a correct explanation for the assertion, the correct option is: - **Option B**: Both assertion and reason are true, but the reason is not a correct explanation of the assertion.
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Read the following statements. Statement A: In agarose gel electrophoresis, the smaller the fragment size of DNA, the farther it moves from the wall. Statement B: Separation of proteins in normal electrophoresis is based on both size and charge. Choose the correct option.

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During gel electrophoresis for separation of DNA fragment :

In gel electrophoresis, separated DNA fragments can be visualized with the help of:

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Write the principle involved in the separation of DNA fragments by gel electrophoresis

Which of the following is correct regarding the separation of DNA fragments during gel electrophoresis?

A: During gel electrophoresis, the DNA fragments move towards the anode. R: DNA fragments are negatively charged molecules .

A: During gel electrophoresis, the DNA fragments move towards the anode. R: DNA fragments are negatively charged molecules .

Assertion : The matrix used in gel electrophoresis should have controllable pore size. Reason : Agarose concentration can be changed to change pore sizes.

ALLEN-BIOTECHNOLOGY AND MICROBES IN HUMAN WELFARE-Exercise IV
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