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Assertion: Presence of restriction sites...

Assertion: Presence of restriction sites of many enzymes within the vecotr is not desirable
Reason :It will generate several fragments which will complicate the process

A

If both Assertion & Reason are True & the Reason is a correct explanation of the Assertion

B

It both Assertion & Reason are True but Reason is not a correct explanation of the Assetion

C

If Assertion is True but the Reason is False

D

If both Assertion & Reason are false

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AI Generated Solution

The correct Answer is:
To solve the question regarding the assertion and reason provided, we will analyze both statements step by step. ### Step-by-Step Solution: 1. **Understanding the Assertion**: - The assertion states that the presence of restriction sites of many enzymes within the vector is not desirable. - This is true because having multiple restriction sites can lead to complications during the cloning process. 2. **Understanding the Reason**: - The reason given is that having multiple restriction sites will generate several fragments, complicating the process. - This is also true, as when multiple restriction enzymes cut the DNA at their respective recognition sites, it results in the formation of several fragments. 3. **Connecting Assertion and Reason**: - The reason explains why the assertion is true. If there are many restriction sites, it indeed generates multiple fragments, making it difficult to isolate the desired fragment for cloning. 4. **Evaluating the Options**: - Since both the assertion and reason are true, and the reason correctly explains the assertion, we conclude that the correct option is: - **Option A**: Both assertion and reason are true, and the reason is a correct explanation of the assertion. ### Conclusion: The answer to the question is **Option A**. ---
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Read the given statements and select the correct option. Statement 1 : The cloning vector is required to have very few, preferably single, recongnition sites for the commonly used restriction enzymes. Statement 2: Presence of more than one recongnition sites within a cloning vector will generate several fragments, which will complicate the process of gene cloining. 1) Both statements 1 and 2 are correct 2) statement 1 is correct but statement 2 is incorrect 3) statement 1 is incorrect but statement 2 is correct 4) None of the above

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ALLEN-BIOTECHNOLOGY AND MICROBES IN HUMAN WELFARE-Exercise IV
  1. Assertion: One can see bright orange coloured band of DNA in a ethidiu...

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  2. Assertion: In Gel electrophorosis, ''the smalller the fragment size, t...

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  3. Assertion: Presence of restriction sites of many enzymes within the ve...

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  4. Assertion: A good vector in that whose ori support high copy number ...

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  5. Assertion : Newer Antibiotics are required to be prosuced Regula...

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  6. Assertion: The inactivation of any vector gene due to insertion of des...

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  7. Assertion: Recombinant colones will produce blue colour in the medium ...

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  8. Assertion: Ti-plasmids & retrovirus are used as vectors in gene transf...

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  9. Assertion: DNA cannot pass through cell membranes Reason :DNA is a h...

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  10. Assertion: Bacterial cells first be made competent by monovalent catio...

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  11. Assertion: In biolistics method, cells are bombarded with high velocit...

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  12. Which technique is used to check the progression of restriction enzyme...

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  13. Assertion: A thermostable DNA polymerase in used in PCR for repeated a...

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  14. Recombinant DNA bearing ampicillin resistance gene is passed in E. col...

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  15. Assertion: The recombinant cells are multiplied in a continuous cultur...

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  16. Assertion: A bioreactor provides the optimal conditions for achieving ...

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  17. Assertion: The most commonly used bioreactors are of stirring type R...

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  18. Assertion: The process including separation & purification of product ...

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  19. Assertion: Genome of an organism can be changed for our benefit by gen...

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  20. Assertion: Polymerase chain reaction is nick named as people's choice ...

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