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Assertion: Recombinant colones will prod...

Assertion: Recombinant colones will produce blue colour in the medium having X-gal compound
Reason :The 'gal gene' of vecotr undergone insertional inactivation

A

If both Assertion & Reason are True & the Reason is a correct explanation of the Assertion

B

It both Assertion & Reason are True but Reason is not a correct explanation of the Assetion

C

If Assertion is True but the Reason is False

D

If both Assertion & Reason are false

Text Solution

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The correct Answer is:
### Step-by-Step Solution: 1. **Understanding the Assertion**: The assertion states that "recombinant colonies will produce blue color in the medium having X-gal compound." In molecular biology, X-gal is used in blue-white screening to differentiate between recombinant and non-recombinant colonies. 2. **Understanding the Reason**: The reason provided is that "the gal gene of the vector underwent insertional inactivation." Insertional inactivation occurs when a foreign DNA is inserted into a gene, disrupting its function. 3. **Analyzing the Role of X-gal**: X-gal is a substrate for the enzyme beta-galactosidase. If the beta-galactosidase is produced (which occurs in non-recombinant colonies), it hydrolyzes X-gal, leading to the formation of a blue pigment. 4. **Determining the Color of Colonies**: - **Non-recombinant colonies** (which have an active beta-galactosidase) will appear blue because they can hydrolyze X-gal. - **Recombinant colonies** (which have undergone insertional inactivation of the gal gene) will not produce beta-galactosidase and will appear white. 5. **Conclusion**: Based on the above analysis: - The assertion that recombinant colonies produce blue color is **false**. - The reason that the gal gene has undergone insertional inactivation is **true**, but it does not correctly explain the assertion. 6. **Final Answer**: Both the assertion and the reason are false.
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ALLEN-BIOTECHNOLOGY AND MICROBES IN HUMAN WELFARE-Exercise IV
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  2. Assertion: The inactivation of any vector gene due to insertion of des...

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  3. Assertion: Recombinant colones will produce blue colour in the medium ...

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  4. Assertion: Ti-plasmids & retrovirus are used as vectors in gene transf...

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  5. Assertion: DNA cannot pass through cell membranes Reason :DNA is a h...

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  6. Assertion: Bacterial cells first be made competent by monovalent catio...

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  7. Assertion: In biolistics method, cells are bombarded with high velocit...

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  8. Which technique is used to check the progression of restriction enzyme...

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  9. Assertion: A thermostable DNA polymerase in used in PCR for repeated a...

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  10. Recombinant DNA bearing ampicillin resistance gene is passed in E. col...

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  11. Assertion: The recombinant cells are multiplied in a continuous cultur...

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  12. Assertion: A bioreactor provides the optimal conditions for achieving ...

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  13. Assertion: The most commonly used bioreactors are of stirring type R...

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