Assertion: The main challenge for production of insulin using r-DNA technique was getting insulin assembled into a mature forms
Reason :The C-peptide is not present in mature insulin & is removed during maturation process

Options

A. If both Assertion & Reason are True & the Reason is a correct explanation of the Assertion
B. It both Assertion & Reason are True but Reason is not a correct explanation of the Assetion
C. If Assertion is True but the Reason is False
D. If both Assertion & Reason are false

Answer

**Step-by-Step Solution:**

1. **Understanding the Assertion**: The assertion states that the main challenge in producing insulin using recombinant DNA (rDNA) technology was getting insulin assembled into its mature form. This implies that the process of maturation is complex and poses difficulties.

2. **Understanding the Reason**: The reason given is that the C-peptide is not present in mature insulin and is removed during the maturation process. This indicates that the C-peptide is a part of the precursor molecule (proinsulin) but is not part of the final product (mature insulin).

3. **Insulin Structure**: Insulin is initially synthesized as proinsulin, which consists of three chains: the A chain, the B chain, and the C-peptide. The A and B chains are linked by disulfide bonds, while the C-peptide connects them.

4. **Maturation Process**: During the maturation process, proinsulin undergoes cleavage where the C-peptide is removed, resulting in the formation of mature insulin, which consists only of the A and B chains.

5. **Challenge in rDNA Technology**: In rDNA technology, producing insulin involves creating the A and B chains separately. The challenge arises because, in a laboratory setting, ensuring the correct cleavage of the C-peptide to form mature insulin is not straightforward. This is a significant hurdle in producing functional insulin.

6. **Conclusion**: Both the assertion and the reason are true. The assertion correctly identifies the challenge of assembling insulin into its mature form, and the reason accurately explains that the removal of the C-peptide is a critical step in this maturation process.

**Final Answer**: Both the assertion and the reason are true, and the reason correctly explains the assertion.

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