Assertion: ADA deficiency can not be cured permanently by gene therapy
Reason :The genetically engineered lymphocytes are immortal only in culture conditions

Options

A. If both Assertion & Reason are True & the Reason is a correct explanation of the Assertion
B. It both Assertion & Reason are True but Reason is not a correct explanation of the Assetion
C. If Assertion is True but the Reason is False
D. If both Assertion & Reason are false

Answer

### Step-by-Step Solution:

1. **Understanding ADA Deficiency**:
- ADA deficiency refers to a lack of the enzyme adenosine deaminase, which is crucial for the immune system. Its deficiency leads to Severe Combined Immunodeficiency (SCID), a serious condition where the immune system is severely compromised.

**Hint**: Recall that ADA stands for adenosine deaminase and is essential for immune function.

2. **Gene Therapy for ADA Deficiency**:
- Gene therapy aims to correct the genetic mutation causing ADA deficiency. By introducing a functional copy of the ADA gene into the patient's cells, the enzyme can be produced, potentially curing SCID.

**Hint**: Think about how gene therapy works to replace or repair defective genes.

3. **Evaluating the Assertion**:
- The assertion states that ADA deficiency cannot be cured permanently by gene therapy. This is incorrect because gene therapy has shown promise in treating this condition effectively, potentially leading to a permanent cure.

**Hint**: Consider the outcomes of gene therapy in clinical trials for ADA deficiency.

4. **Evaluating the Reason**:
- The reason states that genetically engineered lymphocytes are immortal only in culture conditions. This is misleading. While lymphocytes can be cultured indefinitely, successful gene therapy involves their function and survival in the living organism (in vivo), not just in vitro (in culture).

**Hint**: Remember the difference between in vitro (outside a living organism) and in vivo (inside a living organism).

5. **Conclusion**:
- Both the assertion and the reason are false. The assertion incorrectly claims that ADA deficiency cannot be permanently cured by gene therapy, and the reason incorrectly limits the effectiveness of genetically engineered lymphocytes to culture conditions only.

**Hint**: Summarize your findings about the truthfulness of both statements.

### Final Answer:
Both the assertion and the reason are false.