State what happens when an alien gene is ligated at Sal I site of pBR 322 plasmid.

What happens when ? (i)

What happens when dialysis is prolonged?

What happens when alcohol is taken?

State what happens when halogens react with a cold dilute solution of NaOH?

What happens when there is muscular fatigue?

Suppose the gene of interest is inserted as Pstl site in pBR322, the resulting plasmid will confer resistance to :

What happen when iron is treated with: (i). Steam (ii). Dilute HNO_3

Some foreign DNA fragment is attached to Cla I site of pBR 322. This recombinant vector is used to transition Escherichia coli. The cells subjected to transformation are plated on two different media, one containing ampicillin and the other containing tetracycline. The transformed cells containing the recombinant vector will

(a) State what happens in the human body when malarial parasites infected RBCs burst to release the parasites in the blood. (b) Mention the specific sites in the host body where production of (i) sporozoites and (ii) gametocytes take place in the life cycle of the malarial parasites.

If the gene of interest is inserted at the BamHI site in pBR322, the recombinant plasmid will