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(a) State the role of DNA ligase in biot...

(a) State the role of DNA ligase in biotechnology.
(b) What happens when Meloidegyne incognitia consumes cells with RNAi gene?

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Assertion: DNA ligase plays an important role in recombinant DNA technology. Reason: The linking of antibiotic resistance gene with plasmid vector became possible by enzyme DNA ligase

ALLEN-BIOTECHNOLOGY : PRINCIPLES AND PROCESS-Question
  1. Name a few important products of biotechnology

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  2. What is Genetic Engineering?

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  3. (a) State the role of DNA ligase in biotechnology. (b) What happens ...

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  4. How can DNA segment, separated by gel electrophoresis be visualised an...

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  5. List the key tools used in recombinant DNA technology

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  6. What is EcoRI ? How does EcoRI differ form an exonuclease ?

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  7. How is an exonuclease functionally different from an endonuclease and ...

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  8. (a) Mention the difference in the mode of action of exonuclease and en...

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  9. EcoRI is used to cut a segment of foreign DNA and that of a vector DNA...

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  10. (a) Identify (A) and (B) illustrations in the following : Expand ...

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  11. Draw a schematic sketch of pBR 322 plasmid and label the following in ...

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  12. Why is Agrobacterium tumefaciens a good cloning vector ? Explain.

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  13. What are sticky ends and how are they created ?

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  14. What is the process of naming restriction enzymes

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  15. Describe briefly the following (a) Bioreactors (b) Downstream proc...

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  16. Explain briefly : (a) Restriction enzymes (b) Recombination DNA

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  17. What do you understand by "origin of replication" ?

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  18. Amplification of DNA is done by which method during recombinant DNA t...

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  19. Why is Taq polymerase preferred in PCR ? Mention the source of this en...

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  20. (a) Differentiate between exons and introns. (b) What is a plasmid?...

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