Draw a schematic sketch of pBR 322 plasmid and label the following in it:
(a) Any two restriction sites.
(b) Ori and rop genes.
(c) An antibiotic resistant gene.

Which of the following restriction enzyme site is present in nucleotide sequence of ampicillin resistance gene in pBR322?

Draw a diagram to show refraction through a glass slab and label the following: (a) incident ray (b) refracted ray (c) emergent ray (d) angle of incidence (e) angle of refraction at the first boundary of two mediums (f) angle of emergence and (g) lateral displacement

Restriction sites for Pvu I and Pvu II respectively are in which genes of pBR 322

Read the following statements and select the correct option. (I) E.coli cloning vector pBR322 have several restriction sites , ori , antibiotic resistance genes and rop. (II) Competent bacterial cells cannot take up foreign DNA. (III) In elution ,the separated bands of DNA are cut out from agarose gel and extracted from the gel piece. (IV) The downstream processing and quality control testing vary from product to product .

Read the given four statements and choose the correct option. a. An alien DNA is linked with the "ori" for multiplication b. Normal E.coli cells do not carry antibiotic resistant genes for tetracycline and ampicilin c. Salmonella typhimurium carry gene for resistance against antibiotic d. An antibiotic resistance gene can be used as a selectable marker or cloning site

(a) Define excretion. Name any two substances that are selectively reabsorbed. (b) Draw and label the following parts in the human excretory system. (i) Right kidney (ii) Urinary bladder (iii) Right renal artery

State the functions of the following in the cloning vector pBR322 : (i) Ori, (ii) rop, and (iii) Hind III sites.

Read the following sentences : (a) In elution, the separated bands of DNA are cut out from agarose gel & extracted from the gel piece. (b) E coil cloning vector pBR322 have several restriction sites,Ori, antibiotic resistance genes& ROP. (c ) The downstream processing and quality control testing vary from product to product. (d) Competent bacterial cells cannot take up the plasmid.

What could be the possible harm of using antibiotic resistant gene as a selectable marker in plasmid, used for gene transfer in production of GM food? (A) GM food contains the enzyme produced by the antibiotic resistance gene that was used during gene tranfer in genetic engineering. This could cause allergies since it is a foreign protein. (B) The bacteria present in the alimentary canal of the humans could take up the antibiotic resistance gene present in GM food. (C) The bacteria in the human alimentary canal would then become resistant to the concerned antibiotic. (D) The transgene may be transferred through pollen to their wild relatives and make weeds more persistant and damaging.

Select the correct option to fill up balnks. (i) ________is a natural polymer extracted from_____. (ii) The DNA fragments purified by gel electrophoresis are used in constructing______by joining them with_______. (iii) The ligation of alien DNA is carried out at a________. present in one of the two________in a plasmid vector. (iv)_________enzyme remains active during the high temperature induced denaturation of ds DNA (v) DNA fragments are resolved according to their_________ through ______in agarose gel electrophoresis. a) (i) Agarose, sea weeds (ii) recombinant DNA, cloning vector (iii) restriction site, antibiotic resistance genes (iv) Taq polymerase (v) size, sieving effect b) (i) Agarose, sea weeds (ii) Restriction site, antibiotic resistance genes (iii) recombinant DNA, cloning vector (iv) Taq polymerase (v) size, sieving effect c) (i) Agarose, sea weeds (ii) restriction site, antibiotic resistance genes (iii) recombinant DNA, cloning vector (iv) Taq polymerease (v) size, sieving effect d) (i) size, sieving effect (ii) agarose, seaweeds (iii) recombinant DNA cloning vector (iv) Taq polymerase (v) restriction site, antibiotic resistance genes