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What are sticky ends and how are they cr...

What are sticky ends and how are they created ?

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How are 'sticky ends' formed on a DNA strand? Why are they so called ?

How are .sticky ends. formed on a DNA strand? Why are they so called?

Sticky ends, observed in the DNA , are due to the action of a particular type of nucleases. Which of the following statements is correct regarding the sticky ends of the DNA strand ?

Who discovered that restriction enzymes have the capability of cutting DNA strands in a particular fashion, which left what has became known as 'sticky ends' on the strands ?

Assertion: The recombinant DNA molecule cannot be created, unless one cuts the vector & source DNA with same restriction enzyme Reason :When cuts by same enzyme both will have same sticky ends, that can be joined by DNA ligase

Assertion : Restriction enzymes cut the strand of DNA to produce sticky ends. Reason : In this process, there is net gain of twenty four molcules of ATP.

What is variation ? How is variation created in a population ? How does the creation of variation in a species promotes its survival ? (ii) Explain how, offspring and parents of organisms reproducing sexually have the same number of chromosomes.

A polymer is resistant to heat and chemical attack and is also used for coating articles and cookwares to make them non - sticky. The monomer of this polymer is

A recombinant DNA is formed when sticky ends of vector DNA and foreign DNA join. Explain how the sticky ends are formed and get joined.

ALLEN-BIOTECHNOLOGY : PRINCIPLES AND PROCESS-Question
  1. Draw a schematic sketch of pBR 322 plasmid and label the following in ...

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  2. Why is Agrobacterium tumefaciens a good cloning vector ? Explain.

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  3. What are sticky ends and how are they created ?

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  4. What is the process of naming restriction enzymes

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  5. Describe briefly the following (a) Bioreactors (b) Downstream proc...

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  6. Explain briefly : (a) Restriction enzymes (b) Recombination DNA

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  7. What do you understand by "origin of replication" ?

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  8. Amplification of DNA is done by which method during recombinant DNA t...

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  9. Why is Taq polymerase preferred in PCR ? Mention the source of this en...

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  10. (a) Differentiate between exons and introns. (b) What is a plasmid?...

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  11. Recombinant DNA-techology is of great important in the field of medici...

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  12. A sugarcane has been affected by virus. How can a virus free can be de...

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  13. Rearrrange the following in the correct sequence to accomplish an impo...

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  14. (a) Differentiate between exons and introns. (b) What is a plasmid?...

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  15. State the functions of the following in the cloning vector pBR322 : ...

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  16. Explain the basis on which the gel electrophoresis technique works. Wr...

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  17. Can you list 10 recombinant proteins which are used in medical practic...

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  18. Can you think and answer how can a reporter enzyme be used to monitor ...

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  19. (i) Describe theicharacteristics a cloning vector must possess. (ii) W...

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  20. If a desired gene is identified in an organism for some experiments, e...

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