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(i) Describe theicharacteristics a cloni...

(i) Describe theicharacteristics a cloning vector must possess. (ii) Why DNA cannot pass through the cell membrane ? Explain. How is a bacterial cell made competent to take up recombinant DNA from the medium?

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(i) Describe the characteristics a cloning vector must possess. (ii) Why DNA cannot pass through the cell membrane ? Explain. How is a bacterial cell made competent to take up recombinant DNA from the medium?

Assertion: DNA cannot pass through cell membranes Reason :DNA is a hydrophobic molecule

Since DNA is a hydrophilic molecules, it cannot pass through cell membranes. Why ?

DNA being hydrophilic cannot pass through the cell membranes of a hot cell. Explain how does recombinant DNA get introduced into the host cell to transfrom the latter.

DNA being hydrophilic cannot pass through the cell membranes of a hot cell. Explain how does recombinant DNA get introduced into the host cell to transfrom the latter.

How does a bacterial cell protect its own DNA from restriction enzymes?

The bacterial cell made competent to absorb the plasmid DNA by treating with a specific concentration of

Which of the following technique can be used for introduction of hydrophilic recombinant DNA through the cell membranes of a host cell ?

For transformation with recombinant DNA, the bacterial cells must first be made competent which means

The cells of E. coli may be made competent to take up external DNA in the process of transformation by expos- ing the cell to:

ALLEN-BIOTECHNOLOGY : PRINCIPLES AND PROCESS-Question
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  2. Amplification of DNA is done by which method during recombinant DNA t...

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  3. Why is Taq polymerase preferred in PCR ? Mention the source of this en...

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  4. (a) Differentiate between exons and introns. (b) What is a plasmid?...

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  5. Recombinant DNA-techology is of great important in the field of medici...

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  6. A sugarcane has been affected by virus. How can a virus free can be de...

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  7. Rearrrange the following in the correct sequence to accomplish an impo...

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  8. (a) Differentiate between exons and introns. (b) What is a plasmid?...

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  9. State the functions of the following in the cloning vector pBR322 : ...

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  10. Explain the basis on which the gel electrophoresis technique works. Wr...

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  11. Can you list 10 recombinant proteins which are used in medical practic...

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  12. Can you think and answer how can a reporter enzyme be used to monitor ...

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  13. (i) Describe theicharacteristics a cloning vector must possess. (ii) W...

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  14. If a desired gene is identified in an organism for some experiments, e...

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  15. Explain the process involved in the production of recombinant DNA .

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  16. Name and describe that technique that helps in separating the DNA frag...

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  17. Explain the application of biotechnology in producing Bt cotton.

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  18. Unless the vector and source DNA are cut, fragments separated and join...

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  19. Explain the application of rDNA technology to produce insulin.

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  20. (a) Describe the different steps in one complete cycle of PCR. (b) ...

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