Home
Class 12
BIOLOGY
Unless the vector and source DNA are cut...

Unless the vector and source DNA are cut, fragments separated and joined, the desired recombinant vector molecule cannot be created.
(a) How are the desirable DNA sequences cut ?
(b) Explain the technique used to separate the cut fragments.
(c) How are the resultant fragments joined to the vector DNA molecule ?

Promotional Banner

Topper's Solved these Questions

  • BIOTECHNOLOGY : PRINCIPLES & PROCESSES

    ALLEN|Exercise Question|57 Videos

  • BIOTECHNOLOGY : PRINCIPLES AND PROCESSES

    ALLEN|Exercise S|20 Videos

Similar Questions

Explore conceptually related problems

The technique used for separation of DNA fragment is:

Name the technique used for separating DNA fragments in the laboratory.

Name the technique used for separating DNA fragments in the laboratory.

Which of the following recent techniques is used for separating fragments of DNA ?

A fragment of DNA, cut by a restriction enzyme, forms bonds with other DNA molecules that have

Which of the following techniques is used for separation of DNA fragments during DNA fingerprinting?

Name and describe that technique that helps in separating the DNA fragments formed by the use of restriction endonuclease.

Name and describe that technique that helps in separating the DNA fragments formed by the use of restriction endonuclease.

Assertion: The recombinant DNA molecule cannot be created, unless one cuts the vector & source DNA with same restriction enzyme Reason :When cuts by same enzyme both will have same sticky ends, that can be joined by DNA ligase

Name and explain the technique that helps in the separation of DNA fragments for DNA recombinant technology experimants. How can these separated DNA fragments be visualised ?

ALLEN-BIOTECHNOLOGY : PRINCIPLES AND PROCESS-Question
  1. What do you understand by "origin of replication" ?

    Text Solution

    |

  2. Amplification of DNA is done by which method during recombinant DNA t...

    Text Solution

    |

  3. Why is Taq polymerase preferred in PCR ? Mention the source of this en...

    Text Solution

    |

  4. (a) Differentiate between exons and introns. (b) What is a plasmid?...

    Text Solution

    |

  5. Recombinant DNA-techology is of great important in the field of medici...

    Text Solution

    |

  6. A sugarcane has been affected by virus. How can a virus free can be de...

    Text Solution

    |

  7. Rearrrange the following in the correct sequence to accomplish an impo...

    Text Solution

    |

  8. (a) Differentiate between exons and introns. (b) What is a plasmid?...

    Text Solution

    |

  9. State the functions of the following in the cloning vector pBR322 : ...

    Text Solution

    |

  10. Explain the basis on which the gel electrophoresis technique works. Wr...

    Text Solution

    |

  11. Can you list 10 recombinant proteins which are used in medical practic...

    Text Solution

    |

  12. Can you think and answer how can a reporter enzyme be used to monitor ...

    Text Solution

    |

  13. (i) Describe theicharacteristics a cloning vector must possess. (ii) W...

    Text Solution

    |

  14. If a desired gene is identified in an organism for some experiments, e...

    Text Solution

    |

  15. Explain the process involved in the production of recombinant DNA .

    Text Solution

    |

  16. Name and describe that technique that helps in separating the DNA frag...

    Text Solution

    |

  17. Explain the application of biotechnology in producing Bt cotton.

    Text Solution

    |

  18. Unless the vector and source DNA are cut, fragments separated and join...

    Text Solution

    |

  19. Explain the application of rDNA technology to produce insulin.

    Text Solution

    |

  20. (a) Describe the different steps in one complete cycle of PCR. (b) ...

    Text Solution

    |