Draw pBR 322 cloning vector. Lebel 'ORI', _'ROP' and any one antibiotic resistance site on it and state their functions.

Which one of the following characteristics is generally not preferred for a cloning vector ? a) An origin of replication b) An antibiotic resistance marker c)Multiple restriction sites d) A high copy number

Read the following sentences : (a) In elution, the separated bands of DNA are cut out from agarose gel & extracted from the gel piece. (b) E coil cloning vector pBR322 have several restriction sites,Ori, antibiotic resistance genes& ROP. (c ) The downstream processing and quality control testing vary from product to product. (d) Competent bacterial cells cannot take up the plasmid.

P^(BR322) cloning vector has the genes for resistance against

Read the following statements and select the correct option. (I) E.coli cloning vector pBR322 have several restriction sites , ori , antibiotic resistance genes and rop. (II) Competent bacterial cells cannot take up foreign DNA. (III) In elution ,the separated bands of DNA are cut out from agarose gel and extracted from the gel piece. (IV) The downstream processing and quality control testing vary from product to product .

The gene 'rop' present in pBR322 cloning vector, codes for 1) the proteins involved in the translation 2) the proteins involved in the replication of the plasmid 3) the proteins involved in the synthesis of ampicillin only 4) the proteins involved in the synthesis of tetracycline only

If pBR322 cloning vector is cut by Pvu I enzyme for the formation of rDNA then which of its marker gene helps in the selection of recombinants ?

In cloning vector pBR322, the Barn HI cloning site is found in which selectable marker gene?

Select the correct option to fill up balnks. (i) ________is a natural polymer extracted from_____. (ii) The DNA fragments purified by gel electrophoresis are used in constructing______by joining them with_______. (iii) The ligation of alien DNA is carried out at a________. present in one of the two________in a plasmid vector. (iv)_________enzyme remains active during the high temperature induced denaturation of ds DNA (v) DNA fragments are resolved according to their_________ through ______in agarose gel electrophoresis. a) (i) Agarose, sea weeds (ii) recombinant DNA, cloning vector (iii) restriction site, antibiotic resistance genes (iv) Taq polymerase (v) size, sieving effect b) (i) Agarose, sea weeds (ii) Restriction site, antibiotic resistance genes (iii) recombinant DNA, cloning vector (iv) Taq polymerase (v) size, sieving effect c) (i) Agarose, sea weeds (ii) restriction site, antibiotic resistance genes (iii) recombinant DNA, cloning vector (iv) Taq polymerease (v) size, sieving effect d) (i) size, sieving effect (ii) agarose, seaweeds (iii) recombinant DNA cloning vector (iv) Taq polymerase (v) restriction site, antibiotic resistance genes

What will be the effect if pBR322, a cloning vector does not carry 'ori' site ?