Which enzyme links the antibiotic resistance gene with the plasmid vector ?

Answer

### Step-by-Step Solution:

1. **Understanding the Question**: The question asks for the enzyme that links the antibiotic resistance gene with a plasmid vector.

2. **Identifying the Components**:
- The antibiotic resistance gene is typically isolated from a source (like a plasmid).
- The plasmid vector is a circular DNA molecule used to transfer genetic material into a host organism.

3. **Process of Gene Cloning**:
- The antibiotic resistance gene is cut out from its original plasmid using restriction enzymes.
- After isolation, this gene needs to be inserted into a plasmid vector.

4. **Role of Enzymes**:
- The enzyme responsible for linking the antibiotic resistance gene to the plasmid vector is crucial for the formation of recombinant DNA.
- The enzyme that performs this linking function is known as **DNA ligase**.

5. **Conclusion**: Therefore, the enzyme that links the antibiotic resistance gene with the plasmid vector is **DNA ligase**.