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Hind-II always cut DNA molecules at a pa...

Hind-II always cut DNA molecules at a particular point by recognising a specific sequence of six base pairs. This specific base sequence is known as the................ .

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**Step-by-Step Solution:** 1. **Understanding Hind-II**: Hind-II is a type of restriction enzyme, also known as an endonuclease. These enzymes are responsible for cutting DNA at specific locations. 2. **Function of Restriction Enzymes**: Restriction enzymes cut DNA at particular sequences of nucleotides. They can be classified into two types: endonucleases, which cut within the DNA strand, and exonucleases, which cut at the ends of the DNA. 3. **Specificity of Hind-II**: Hind-II recognizes a specific sequence of six base pairs in the DNA. This specificity is crucial for its function as a restriction enzyme. 4. **Identifying the Sequence**: The specific base sequence recognized by Hind-II starts with the nucleotide sequence 5' G T, followed by a pyrimidine (either cytosine or thymine), then a purine (adenine or guanine), and ends with A and C. The complementary strand would have the sequence that pairs with this. 5. **Terminology**: The specific sequence of base pairs that Hind-II recognizes and cuts is referred to as the "recognition sequence." **Final Answer**: The specific base sequence is known as the **recognition sequence**. ---
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ALLEN-BIOTECHNOLOGY : PRINCIPLES & PROCESSES-Question
  1. Name the enzyme that cut the DNA at specific site :-

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  2. The first discovered restriction endonuclease enzyme was :-

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  3. Hind-II always cut DNA molecules at a particular point by recognising ...

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  4. Today we know more than that have been isolated from overof bacteria e...

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  5. Match the column for enzyme EcoRI

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  6. Restriction enzymes belong to which class of enzymes ?

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  7. An enzyme catalysing the removal of nucleotides from the ends of DNA i...

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  8. Read the given statements and select the correct option, Statement 1...

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  9. What is the Recognition sequence of EcoRI ?

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  10. Identify the A, B, C and D in given diagram.

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  11. The palindromes are groups of letters that form the …….when read in bo...

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  12. What is the process of insertion of recombinant DNA in bacterial cell ...

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  13. The cell which contain recombinant DNA

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  14. Name the technique used for separating DNA fragments in the laboratory...

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  15. In electrophoresis which compound is use to stain DNA fragments

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  16. Identify the A and B in given diagram.

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  17. Now a days which is the most commonly used matrix of electrophoresis ?

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  18. From where the agarose extracted ?

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  19. Name the useful selectable markers for E.coli ?

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  20. Identify the A, B, C, D, E and F in the given diagram

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