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What is the process of insertion of reco...

What is the process of insertion of recombinant DNA in bacterial cell ?

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Transformation
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What is recombinant DNA?

In the process of insertional inactivation 1) a recombinant DNA is inserted within the coding sequence of enzyme β -galactosidase, resulting in inactivation of the enzyme 2) a recombinant DNA is inserted within the coding sequence of proteins involved in the replication of the plasmid 3) a recombinat DNA is inserted within the recongnition site for EcoRl 4) none of these

Explain the process involved in the production of recombinant DNA .

Synthesis of recombinant DNA requires :

Which is not a method for the introduction of recombinant DNA into host cells?

Which of the following can be used to insert the recombinant DNA into the animal cell?

The cell which contain recombinant DNA

The different steps of recombinant DNA technology are given below randomly. (i) Isolation of the DNA fragments or genes to be cloned (ii) Introduction of the recombinant DNA into a suitable cell (usually E. coil) called host (transformation) (iii) Multiplication/expression of the introduced gene in the host (iv) Selection of the transformed host cells, and identification of the clone containing the desired gene/DNA fragment (v) Insertion of the isolated gene in a suitable plasmid vector Which of the following represents the correct sequences of steps ?

For transformation with recombinant DNA, the bacterial cells must first be made competent which means

In an experiment of recombinant DNA technology, in a bacterial culture some of the colonies produced blue colour in presence of a chromogenic substrate. Why?

ALLEN-BIOTECHNOLOGY : PRINCIPLES & PROCESSES-Question
  1. Identify the A, B, C and D in given diagram.

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  2. The palindromes are groups of letters that form the …….when read in bo...

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  3. What is the process of insertion of recombinant DNA in bacterial cell ...

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  4. The cell which contain recombinant DNA

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  5. Name the technique used for separating DNA fragments in the laboratory...

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  6. In electrophoresis which compound is use to stain DNA fragments

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  7. Identify the A and B in given diagram.

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  8. Now a days which is the most commonly used matrix of electrophoresis ?

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  9. From where the agarose extracted ?

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  10. Name the useful selectable markers for E.coli ?

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  11. Identify the A, B, C, D, E and F in the given diagram

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  12. The extraction of DNA fragment from agarose gel is called .

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  13. Match the column

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  14. Name the process which is use to check the progression of restriction ...

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  15. (a) A recombinant vector with a gene of interest inserted within the g...

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  16. True and false : The uptake of DNA furing transformation is an activ...

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  17. In the process of insertional inactivation

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  18. True or false : Bioreactor provides the optimal conditions for obtai...

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  19. True and false : In insertional inactivation process alien gene intr...

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  20. The process include separation and purification of desirable protein i...

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