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Name the process which is use to check the progression of restriction enzyme digestion.

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**Step-by-Step Solution:** 1. **Understanding Restriction Enzyme Digestion**: Restriction enzymes, often referred to as molecular scissors, cut DNA at specific sequences. This process results in the generation of DNA fragments of varying lengths. 2. **Identifying the Need for Monitoring**: After performing restriction enzyme digestion, it is essential to check whether the digestion has occurred successfully and to what extent the DNA has been cut into fragments. 3. **Introducing the Technique**: The technique used to monitor the progression of restriction enzyme digestion is called agarose gel electrophoresis. 4. **How Agarose Gel Electrophoresis Works**: In this technique, the digested DNA fragments are loaded into an agarose gel. When an electric current is applied, the negatively charged DNA fragments migrate towards the positively charged electrode (anode). 5. **Separation of DNA Fragments**: The movement of the DNA fragments through the gel matrix allows for the separation based on size. Smaller fragments move faster and farther through the gel, while larger fragments move more slowly and remain closer to the starting point. 6. **Interpreting the Results**: By analyzing the pattern of the DNA fragments on the gel, one can determine if the restriction enzyme digestion has occurred. If there are visible bands representing DNA fragments, it indicates successful digestion. If no bands are present, it suggests that digestion has not taken place. 7. **Conclusion**: Therefore, the process used to check the progression of restriction enzyme digestion is agarose gel electrophoresis. ---
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Read the following statements regarding agarose gel electrophoresis and select the CORRECT ones. I. The longest DNA fragments are nearest to the anode. II. The matrix of the gel is a natural polymer obtained from seaweeds. III The process is used to check the progression of restriction enzyme digestion. IV. The fragments of DNA separated due to the sieving effect provided by agarose gel.

Which technique is used to check the progression of restriction enzyme digestion

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Which of the following techniques is used to check the progression of restriction enzyme digestion ?

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Which one in not a restriction enzyme :

Type - II restriction enzymes

ALLEN-BIOTECHNOLOGY : PRINCIPLES & PROCESSES-Question
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