(a) A recombinant vector with a gene of ...

(a) A recombinant vector with a gene of interest inserted within the gene of a-galactosidase enzyme, is introduced into a bacterium. Explain the method that would help in selection of recombinant colonies from non-recombinant ones.
(b) Why is this method of selection referred to as''insertional inactivation''?

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Cromogenic substrate

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(a) A recombinant vector with a gene of interest inserted within the gene of beta-galactosidase enzyme, is introduced into a bacterium. Explain the method that would help in selection of recombinant colonies from non-recombinant ones. (b) Why is this method of selection referred to as ''insertional inactivation''?

How is insertional inactivation of an enzyme used as a selectable marker to differentiate recombinants from non-recombinants?

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Assertion: Recombinant colones will produce blue colour in the medium having X-gal compound Reason :The 'gal gene' of vecotr undergone insertional inactivation

If a person obtains transformants by inserting a recombinant DNA within the coding sequence of enzyme beta-galatosidase, he will separate out recombinants from non-recombinats by which of the following observations ?

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Insertional inactivation is related to: (a) Microinjection (b) Gene gun (c) Gel electrophoresis (d) Selection of recombinants

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Read the given statements and select the correct option Statement 1 : In insertional inactivation, blue colour produced by bacterial colonies indicates that the plasmid does not have an insert into the bacterial genome. Statement 2: Presence of insert results into insertional inactivation of beta -galactosidase enzyme and the colonies do not produce any colour. A) Both statements 1 and 2 are correct B) statement 1 is correct but statement 2 is incorrect C) statement 1 is incorrect but statement 2 is correct D) Both statements 1 and 2 are incorrect

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