In the process of insertional inactivati...

In the process of insertional inactivation

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To which of the following techniques would you associ- ate the phenomenon of insertional inactivation?

What is insertional inactivation?

Read the given statements and select the correct option Statement 1 : In insertional inactivation, blue colour produced by bacterial colonies indicates that the plasmid does not have an insert into the bacterial genome. Statement 2: Presence of insert results into insertional inactivation of beta -galactosidase enzyme and the colonies do not produce any colour. A) Both statements 1 and 2 are correct B) statement 1 is correct but statement 2 is incorrect C) statement 1 is incorrect but statement 2 is correct D) Both statements 1 and 2 are incorrect

For selection of recombinants, insertional inactivation of antibiotic marker has been superseded by insertional inactivation of a marker gene coding for a chromogenic substrate. Give reasons.

Assertion: The inactivation of any vector gene due to insertion of desired gene called as insertional inactivation Reason :Some time restriction sites are also present in between the gene sequence of vector

The presence of a chromogenic substrate gives blue coloured colonies of bacteria. Insertional inactivation would stop product of which of the following enzyme such that colonies do not produce any colour.

Assetion: Insertion of recombinant DNA within the coding sequence of beta- galactosidase result n colourless colonies. Reason : Presence of insert results in inactivation of enzyme beta- galactosidase known as insertional inactivation

Assertion: Recombinant colones will produce blue colour in the medium having X-gal compound Reason :The 'gal gene' of vecotr undergone insertional inactivation

True and false : In insertional inactivation process alien gene introduced in ori. Sequence.

ALLEN-BIOTECHNOLOGY : PRINCIPLES & PROCESSES-Question

The extraction of DNA fragment from agarose gel is called .
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Match the column
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Name the process which is use to check the progression of restriction ...
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(a) A recombinant vector with a gene of interest inserted within the g...
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True and false : The uptake of DNA furing transformation is an activ...
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In the process of insertional inactivation
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True or false : Bioreactor provides the optimal conditions for obtai...
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True and false : In insertional inactivation process alien gene intr...
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The process include separation and purification of desirable protein i...
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After insertion inactivation of galactosidase gene which type of colon...
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The part of Ti Plasmid is transferred by Agrobacterium in dicot plant ...
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If any protein encoding gene is expressed in a heterologus host, is ca...
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Which type of ion use to increase the efficiency uptake of DNA by bact...
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The bacterial cell which is able to uptake plasmid DNA is called .
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In biolistic or gene gun method DNA is coated with .
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By which method rDNA is directly injected into the nucleus of animal c...
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Which enzyme is use for removal of RNA molecule in DNA isolation proce...
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During isolation of genetic material, the chemical used to precipitate...
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Identify A, B, C and D in given diagram
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From which bacteria thermo stable taq DNA polymerase is isolated ?
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