True and false :
In insertional inactivation process alien gene introduced in ori. Sequence.

In insertional inactivation of beta -galactosidase gene, the bacteria in white colonies have

In the process of insertional inactivation 1) a recombinant DNA is inserted within the coding sequence of enzyme β -galactosidase, resulting in inactivation of the enzyme 2) a recombinant DNA is inserted within the coding sequence of proteins involved in the replication of the plasmid 3) a recombinat DNA is inserted within the recongnition site for EcoRl 4) none of these

For selection of recombinants, insertional inactivation of antibiotic marker has been superseded by insertional inactivation of a marker gene coding for a chromogenic substrate. Give reasons.

After insertion inactivation of galactosidase gene which type of colonies do not produce any colour ?

Assertion: The inactivation of any vector gene due to insertion of desired gene called as insertional inactivation Reason :Some time restriction sites are also present in between the gene sequence of vector

True and false : The uptake of DNA furing transformation is an active process.

The different steps of recombinant DNA technology are given below randomly. (i) Isolation of the DNA fragments or genes to be cloned (ii) Introduction of the recombinant DNA into a suitable cell (usually E. coil) called host (transformation) (iii) Multiplication/expression of the introduced gene in the host (iv) Selection of the transformed host cells, and identification of the clone containing the desired gene/DNA fragment (v) Insertion of the isolated gene in a suitable plasmid vector Which of the following represents the correct sequences of steps ?