If a re combinant DNA bearing ge ne for tetracyclin resistance is transferred into E.coli cells and the host cells are spread on agar plates containing tetracyclin then :-

Options

A. Both transformed and non-transforme recipient cells will die
B. Both transformed and non transformed cells will grow
C. Transformed recipient cells will die and non transformed recipient cells will grow
D. Transformed recipient cells will grow and non-transformed recipient cells will die

Answer

To solve the question regarding the transfer of recombinant DNA bearing a gene for tetracycline resistance into E. coli cells, we can break down the process into clear steps:

### Step-by-Step Solution:

1. **Understanding the Components**:
- We have recombinant DNA that includes a gene for tetracycline resistance.
- This DNA is transferred into E. coli cells.

2. **Transformation Process**:
- Some E. coli cells successfully take up the recombinant DNA and become "transformants."
- Other E. coli cells do not take up the DNA and remain "non-transformants."

3. **Setting Up the Experiment**:
- The transformed and non-transformed E. coli cells are spread on agar plates that contain tetracycline, an antibiotic.

4. **Effect of Tetracycline**:
- Tetracycline will inhibit the growth of bacteria that do not have the resistance gene.
- The non-transformants, which lack the gene for tetracycline resistance, will die in the presence of the antibiotic.

5. **Survival of Transformants**:
- The transformants, which have taken up the recombinant DNA with the tetracycline resistance gene, will survive and grow on the agar plates.

6. **Conclusion**:
- Therefore, on the agar plates containing tetracycline, the transformed E. coli cells will grow while the non-transformed cells will die.

### Final Answer:
The correct conclusion is that **transformed recipient cells will grow and non-transformed recipient cells will die**.